MOSQUITOR HOUSE

MOSQUITO HOUSE/ANIMAL HOUSE/INSECTARY

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Name of project: Other entomology studies/activities
Overall Principal Investigator: Dr Monicah Mburu
Local Study Coordinator: LimontySimubali

Insectary
The three insectaries (referred to as A, B and C) have been active throughout the reporting period. Rearing of a mosquito colony i.e. An. gambiae (Kisumu strain)has been going on in insectaries B and C. The productivity of mosquitoes from these insectaries has been meeting the demand for mosquitoes required for bioassays. We introduced a new blood-feeding method for mosquitoes to complement the usual blood feeding method that has been in place. The new method is referred to as ‘consecutive blood-feeding’ method. Through this method, the mosquitoes receive enough blood-meals resulting in large batches of mosquito eggs, hence a more stable colony. We assisted the United States’ Presidential Malaria Initiative (PMI)VectorLink at National Malaria Elimination Center (NMEC) in Lusaka who requested for some mosquito eggs and adult mosquitoes to boost their mosquito colony. Insectary A serves as a room for the bioassays with the same mosquitoes. The bioassays include those of the ATSB project.

Animal house
The animal house accommodates mice that are used for provision of blood-meals to mosquitoes in the insectaries. This helps in the sustenance of the mosquito colony. The health of the mice is always monitored. There have been no challenges during the reporting period. Plans are underway to reduce inbreeding of the mice.

Mosquito house (greenhouse-like structure)
The mosquito house serves as a large semi-field structure. The mosquito house has six chambers. The netting that surrounds the mosquito house has been newly replaced as well as the grass on the ceiling. It is now ready for experiments such as those of super attractive lures project.
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